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smad5 expression  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology smad5 expression
    Smad5 Expression, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smad5+expression/Smad5+siRNA/10__1161_slash_circulationaha__111__060269-448-34-37
    Average 93 stars, based on 12 article reviews
    smad5 expression - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: MicroRNA-21 Integrates Pathogenic Signaling to Control Pulmonary Hypertension
    Article Snippet: For miRNA inhibition, hairpin loop oligonucleotides (Dharmacon) that carry specific antisense sequences to miR-21 (AS-21) or control (AS-Cont, 40-80 nM) were similarly transfected. .. Validated inhibitory RNA were used for RNA interference of BMPRII expression (siRNA VHS41350, Invitrogen, 80 nM transfection) and SMAD4 expression (siRNA VHS41118, Invitrogen, 80 nM transfection); additional inhibitory RNA were used for interference of SMAD5 expression (sc-38378, Santa Cruz Biotechnology, 80 nM transfection) and AKT2 expression (sc-29197, Santa Cruz Biotechnology, 80 nM transfection). .. A combination of three siRNA controls with matching GC content was used as a negative control (RNAi Negative Control LO GC, 80 nM, Invitrogen).

    Transfection:

    Article Title: MicroRNA-21 Integrates Pathogenic Signaling to Control Pulmonary Hypertension
    Article Snippet: For miRNA inhibition, hairpin loop oligonucleotides (Dharmacon) that carry specific antisense sequences to miR-21 (AS-21) or control (AS-Cont, 40-80 nM) were similarly transfected. .. Validated inhibitory RNA were used for RNA interference of BMPRII expression (siRNA VHS41350, Invitrogen, 80 nM transfection) and SMAD4 expression (siRNA VHS41118, Invitrogen, 80 nM transfection); additional inhibitory RNA were used for interference of SMAD5 expression (sc-38378, Santa Cruz Biotechnology, 80 nM transfection) and AKT2 expression (sc-29197, Santa Cruz Biotechnology, 80 nM transfection). .. A combination of three siRNA controls with matching GC content was used as a negative control (RNAi Negative Control LO GC, 80 nM, Invitrogen).



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    Fig. 1. Effect of <t>SMAD5</t> knockdown on osteogenic differentiation of BMSCs. (A) WB analysis was used to detect SMAD5 protein levels in BMSCs cultured with GM and OM at days 0, 6 and 12. (B–I) BMSCs were transfected with sh-NC/sh-SMAD5 and then induced with OM. (B) SMAD5 protein level was tested by WB analysis. (C) Cell viability was assayed using CCK8. (D, E) Cell invasion was assessed by transwell assay. (F) ALP activity was measured by ALP Assay Kit. (G, H) ARS staining was performed to examine mineralization levels. (I) The protein expression levels of OCN, Runx2, OPN and BSP were detected by WB analysis. *P < 0.05.
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    Fig. 1. Effect of <t>SMAD5</t> knockdown on osteogenic differentiation of BMSCs. (A) WB analysis was used to detect SMAD5 protein levels in BMSCs cultured with GM and OM at days 0, 6 and 12. (B–I) BMSCs were transfected with sh-NC/sh-SMAD5 and then induced with OM. (B) SMAD5 protein level was tested by WB analysis. (C) Cell viability was assayed using CCK8. (D, E) Cell invasion was assessed by transwell assay. (F) ALP activity was measured by ALP Assay Kit. (G, H) ARS staining was performed to examine mineralization levels. (I) The protein expression levels of OCN, Runx2, OPN and BSP were detected by WB analysis. *P < 0.05.
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    Fig. 1. Effect of <t>SMAD5</t> knockdown on osteogenic differentiation of BMSCs. (A) WB analysis was used to detect SMAD5 protein levels in BMSCs cultured with GM and OM at days 0, 6 and 12. (B–I) BMSCs were transfected with sh-NC/sh-SMAD5 and then induced with OM. (B) SMAD5 protein level was tested by WB analysis. (C) Cell viability was assayed using CCK8. (D, E) Cell invasion was assessed by transwell assay. (F) ALP activity was measured by ALP Assay Kit. (G, H) ARS staining was performed to examine mineralization levels. (I) The protein expression levels of OCN, Runx2, OPN and BSP were detected by WB analysis. *P < 0.05.
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    Fig. 1. Effect of SMAD5 knockdown on osteogenic differentiation of BMSCs. (A) WB analysis was used to detect SMAD5 protein levels in BMSCs cultured with GM and OM at days 0, 6 and 12. (B–I) BMSCs were transfected with sh-NC/sh-SMAD5 and then induced with OM. (B) SMAD5 protein level was tested by WB analysis. (C) Cell viability was assayed using CCK8. (D, E) Cell invasion was assessed by transwell assay. (F) ALP activity was measured by ALP Assay Kit. (G, H) ARS staining was performed to examine mineralization levels. (I) The protein expression levels of OCN, Runx2, OPN and BSP were detected by WB analysis. *P < 0.05.

    Journal: Scientific reports

    Article Title: EIF4A3 enhances the viability, invasion and osteogenic differentiation of BMSCs via the USP53/SMAD5 pathway.

    doi: 10.1038/s41598-025-86048-1

    Figure Lengend Snippet: Fig. 1. Effect of SMAD5 knockdown on osteogenic differentiation of BMSCs. (A) WB analysis was used to detect SMAD5 protein levels in BMSCs cultured with GM and OM at days 0, 6 and 12. (B–I) BMSCs were transfected with sh-NC/sh-SMAD5 and then induced with OM. (B) SMAD5 protein level was tested by WB analysis. (C) Cell viability was assayed using CCK8. (D, E) Cell invasion was assessed by transwell assay. (F) ALP activity was measured by ALP Assay Kit. (G, H) ARS staining was performed to examine mineralization levels. (I) The protein expression levels of OCN, Runx2, OPN and BSP were detected by WB analysis. *P < 0.05.

    Article Snippet: Besides, BMSCs transfected with sh-NC/shUSP53 and then treated with proteasome inhibitor MG132 (10 μmol/L; HY-13259, MedChemExpress) for 24 h to explore whether USP53 regulated SMAD5 expression through deubiquitinating.

    Techniques: Knockdown, Cell Culture, Transfection, Transwell Assay, Activity Assay, ALP Assay, Staining, Expressing

    Fig. 2. Effect of SMAD5 overexpression on osteogenic differentiation of BMSCs. BMSCs were transfected with pcDNA/SMAD5 overexpression vectors and then induced with OM. (A) SMAD5 protein level was tested by WB analysis. Cell viability, invasion, ALP activity, mineralization levels, and the protein levels of OCN, Runx2, OPN and BSP were measured using CCK8 (B), transwell assay (C), ALP Assay Kit (D), ARS staining (E) and WB analysis (F). *P < 0.05.

    Journal: Scientific reports

    Article Title: EIF4A3 enhances the viability, invasion and osteogenic differentiation of BMSCs via the USP53/SMAD5 pathway.

    doi: 10.1038/s41598-025-86048-1

    Figure Lengend Snippet: Fig. 2. Effect of SMAD5 overexpression on osteogenic differentiation of BMSCs. BMSCs were transfected with pcDNA/SMAD5 overexpression vectors and then induced with OM. (A) SMAD5 protein level was tested by WB analysis. Cell viability, invasion, ALP activity, mineralization levels, and the protein levels of OCN, Runx2, OPN and BSP were measured using CCK8 (B), transwell assay (C), ALP Assay Kit (D), ARS staining (E) and WB analysis (F). *P < 0.05.

    Article Snippet: Besides, BMSCs transfected with sh-NC/shUSP53 and then treated with proteasome inhibitor MG132 (10 μmol/L; HY-13259, MedChemExpress) for 24 h to explore whether USP53 regulated SMAD5 expression through deubiquitinating.

    Techniques: Over Expression, Transfection, Activity Assay, Transwell Assay, ALP Assay, Staining

    Fig. 3. Effect of USP53 on SMAD5 expression. (A) The ubibrowser website revealed the regulation of USP53 on SMAD5. (B–D) BMSCs were transfected with sh-NC/sh-USP53. (B) USP53 protein level was tested by WB analysis. (D) qRT-PCR and WB analysis were used to detect SMAD5 mRNA and protein levels. (E) Co-IP was performed to measure the interaction between USP53 and SMAD5. (F) SMAD5 protein level was detected by WB analysis in BMSCs transfected with pcDNA/USP53 overexpression vector and treated with CHX at 0, 3, 6, 9, and 12 h. (G) SMAD5 protein level was tested using WB analysis in BMSCs transfected with sh-NC/ sh-USP53 and treated with MG132. (H) Ubiquitination analysis was performed in BMSCs transfected with or without sh-NC/sh-USP53. *P < 0.05.

    Journal: Scientific reports

    Article Title: EIF4A3 enhances the viability, invasion and osteogenic differentiation of BMSCs via the USP53/SMAD5 pathway.

    doi: 10.1038/s41598-025-86048-1

    Figure Lengend Snippet: Fig. 3. Effect of USP53 on SMAD5 expression. (A) The ubibrowser website revealed the regulation of USP53 on SMAD5. (B–D) BMSCs were transfected with sh-NC/sh-USP53. (B) USP53 protein level was tested by WB analysis. (D) qRT-PCR and WB analysis were used to detect SMAD5 mRNA and protein levels. (E) Co-IP was performed to measure the interaction between USP53 and SMAD5. (F) SMAD5 protein level was detected by WB analysis in BMSCs transfected with pcDNA/USP53 overexpression vector and treated with CHX at 0, 3, 6, 9, and 12 h. (G) SMAD5 protein level was tested using WB analysis in BMSCs transfected with sh-NC/ sh-USP53 and treated with MG132. (H) Ubiquitination analysis was performed in BMSCs transfected with or without sh-NC/sh-USP53. *P < 0.05.

    Article Snippet: Besides, BMSCs transfected with sh-NC/shUSP53 and then treated with proteasome inhibitor MG132 (10 μmol/L; HY-13259, MedChemExpress) for 24 h to explore whether USP53 regulated SMAD5 expression through deubiquitinating.

    Techniques: Expressing, Transfection, Quantitative RT-PCR, Co-Immunoprecipitation Assay, Over Expression, Plasmid Preparation, Ubiquitin Proteomics

    Fig. 4. Effect of USP53 and SMAD5 on osteogenic differentiation of BMSCs. BMSCs were co-transfected with sh-NC/sh-USP53 and pcDNA/SMAD5 overexpression vector in OM. (A) WB analysis was used to test SMAD5 protein level. CCK8 (B), transwell assay (C), ALP Assay Kit (D), ARS staining (E, F) and WB analysis (G) were performed to measure cell viability, invasion, ALP activity, mineralization levels, and the protein levels of OCN, Runx2, OPN and BSP. *P < 0.05.

    Journal: Scientific reports

    Article Title: EIF4A3 enhances the viability, invasion and osteogenic differentiation of BMSCs via the USP53/SMAD5 pathway.

    doi: 10.1038/s41598-025-86048-1

    Figure Lengend Snippet: Fig. 4. Effect of USP53 and SMAD5 on osteogenic differentiation of BMSCs. BMSCs were co-transfected with sh-NC/sh-USP53 and pcDNA/SMAD5 overexpression vector in OM. (A) WB analysis was used to test SMAD5 protein level. CCK8 (B), transwell assay (C), ALP Assay Kit (D), ARS staining (E, F) and WB analysis (G) were performed to measure cell viability, invasion, ALP activity, mineralization levels, and the protein levels of OCN, Runx2, OPN and BSP. *P < 0.05.

    Article Snippet: Besides, BMSCs transfected with sh-NC/shUSP53 and then treated with proteasome inhibitor MG132 (10 μmol/L; HY-13259, MedChemExpress) for 24 h to explore whether USP53 regulated SMAD5 expression through deubiquitinating.

    Techniques: Transfection, Over Expression, Plasmid Preparation, Transwell Assay, ALP Assay, Staining, Activity Assay

    Fig. 5. Effect of EIF4A3 on USP53 expression. (A) The ENCORI websites analyzed the interaction of USP53 and EIF4A3. (B) The interaction between USP53 and EIF4A3 was detected by Co-IP. (C) BMSCs were transfected with sh-NC/sh-EIF4A3/pcDNA/EIF4A3 overexpression vector, and WB analysis was used to determine EIF4A3 protein level. (D, E) Act D assay was performed to measure the stability of USP53 mRNA. (F) WB analysis for the detection of USP53 protein expression. *P < 0.05.

    Journal: Scientific reports

    Article Title: EIF4A3 enhances the viability, invasion and osteogenic differentiation of BMSCs via the USP53/SMAD5 pathway.

    doi: 10.1038/s41598-025-86048-1

    Figure Lengend Snippet: Fig. 5. Effect of EIF4A3 on USP53 expression. (A) The ENCORI websites analyzed the interaction of USP53 and EIF4A3. (B) The interaction between USP53 and EIF4A3 was detected by Co-IP. (C) BMSCs were transfected with sh-NC/sh-EIF4A3/pcDNA/EIF4A3 overexpression vector, and WB analysis was used to determine EIF4A3 protein level. (D, E) Act D assay was performed to measure the stability of USP53 mRNA. (F) WB analysis for the detection of USP53 protein expression. *P < 0.05.

    Article Snippet: Besides, BMSCs transfected with sh-NC/shUSP53 and then treated with proteasome inhibitor MG132 (10 μmol/L; HY-13259, MedChemExpress) for 24 h to explore whether USP53 regulated SMAD5 expression through deubiquitinating.

    Techniques: Expressing, Co-Immunoprecipitation Assay, Transfection, Over Expression, Plasmid Preparation

    Fig. 6. Effect of EIF4A3 and USP53 on osteogenic differentiation of BMSCs. BMSCs were co-transfected with sh-NC/sh-EIF4A3 and pcDNA/USP53 overexpression vector in OM. (A) USP53 protein level was detected by WB analysis. Cell viability and invasion were measured by CCK8 (B) and transwell assay (C). (D) ALP Assay Kit was used to tested ALP activity. (E–G) Mineralization levels and the protein levels of OCN, Runx2, OPN and BSP were measured using ARS staining and WB analysis. *P < 0.05.

    Journal: Scientific reports

    Article Title: EIF4A3 enhances the viability, invasion and osteogenic differentiation of BMSCs via the USP53/SMAD5 pathway.

    doi: 10.1038/s41598-025-86048-1

    Figure Lengend Snippet: Fig. 6. Effect of EIF4A3 and USP53 on osteogenic differentiation of BMSCs. BMSCs were co-transfected with sh-NC/sh-EIF4A3 and pcDNA/USP53 overexpression vector in OM. (A) USP53 protein level was detected by WB analysis. Cell viability and invasion were measured by CCK8 (B) and transwell assay (C). (D) ALP Assay Kit was used to tested ALP activity. (E–G) Mineralization levels and the protein levels of OCN, Runx2, OPN and BSP were measured using ARS staining and WB analysis. *P < 0.05.

    Article Snippet: Besides, BMSCs transfected with sh-NC/shUSP53 and then treated with proteasome inhibitor MG132 (10 μmol/L; HY-13259, MedChemExpress) for 24 h to explore whether USP53 regulated SMAD5 expression through deubiquitinating.

    Techniques: Transfection, Over Expression, Plasmid Preparation, Transwell Assay, ALP Assay, Activity Assay, Staining

    Fig. 7. EIF4A3 affected SMAD5 expression via mediating USP53. (A) WB analysis was used to measure SMAD5 protein level in BMSCs co-transfected with sh-NC/sh-EIF4A3 and pcDNA/USP53 overexpression vector. (B) Summary diagram of this study. *P < 0.05.

    Journal: Scientific reports

    Article Title: EIF4A3 enhances the viability, invasion and osteogenic differentiation of BMSCs via the USP53/SMAD5 pathway.

    doi: 10.1038/s41598-025-86048-1

    Figure Lengend Snippet: Fig. 7. EIF4A3 affected SMAD5 expression via mediating USP53. (A) WB analysis was used to measure SMAD5 protein level in BMSCs co-transfected with sh-NC/sh-EIF4A3 and pcDNA/USP53 overexpression vector. (B) Summary diagram of this study. *P < 0.05.

    Article Snippet: Besides, BMSCs transfected with sh-NC/shUSP53 and then treated with proteasome inhibitor MG132 (10 μmol/L; HY-13259, MedChemExpress) for 24 h to explore whether USP53 regulated SMAD5 expression through deubiquitinating.

    Techniques: Expressing, Transfection, Over Expression, Plasmid Preparation